CD5 levels define functionally heterogeneous populations of naïve human CD4+ T cells
A Sood, MÈ Lebel, M Dong, M Fournier… - European Journal of …, 2021 - Wiley Online Library
European Journal of Immunology, 2021•Wiley Online Library
Studies in murine models show that subthreshold TCR interactions with self‐peptide are
required for thymic development and peripheral survival of naïve T cells. Recently,
differences in the strength of tonic TCR interactions with self‐peptide, as read‐out by cell
surface levels of CD5, were associated with distinct effector potentials among sorted
populations of T cells in mice. However, whether CD5 can also be used to parse functional
heterogeneity among human T cells is less clear. Our study demonstrates that CD5 levels …
required for thymic development and peripheral survival of naïve T cells. Recently,
differences in the strength of tonic TCR interactions with self‐peptide, as read‐out by cell
surface levels of CD5, were associated with distinct effector potentials among sorted
populations of T cells in mice. However, whether CD5 can also be used to parse functional
heterogeneity among human T cells is less clear. Our study demonstrates that CD5 levels …
Abstract
Studies in murine models show that subthreshold TCR interactions with self‐peptide are required for thymic development and peripheral survival of naïve T cells. Recently, differences in the strength of tonic TCR interactions with self‐peptide, as read‐out by cell surface levels of CD5, were associated with distinct effector potentials among sorted populations of T cells in mice. However, whether CD5 can also be used to parse functional heterogeneity among human T cells is less clear. Our study demonstrates that CD5 levels correlate with TCR signal strength in human naïve CD4+ T cells. Further, we describe a relationship between CD5 levels on naïve human CD4+ T cells and binding affinity to foreign peptide, in addition to a predominance of CD5hi T cells in the memory compartment. Differences in gene expression and biases in cytokine production potential between CD5lo and CD5hi naïve human CD4+ T cells are consistent with observations in mice. Together, these data validate the use of CD5 surface levels as a marker of heterogeneity among human naïve CD4+ T cells with important implications for the identification of functionally biased T‐ cell populations that can be exploited to improve the efficacy of adoptive cell therapies.
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